Kinetic studies of ceruloplasmin-azide interaction
نویسندگان
چکیده
منابع مشابه
comparative dna interaction studies of antiviral drug, zidovudine and its complex using different instrumental methods
هدف از این مطالعه بررسی امکان استفاده از داروهای شناخته شده در درمان سایر بیماریها به عنوان داروهای ضد سرطان است. همچنین با استفاده از این داروها در ساختمان کمپلکس فلز می توان شاخص های دارویی بدست آمده را بررسی نمود. داروی ضد ویروس ایدز(hiv)به نام زیدوودین(azt)انتخاب و.کمپلکس.محلول.در.آب[pt(azt)2]cl2سنتزو به روشهای مختلف فیزیکی و شیمیایی شناسایی گردید. بر هم کنش مقایسه ای این دارو و کمپلکس پلا...
15 صفحه اولKinetic studies of ferrous ion oxidation with crystalline human ferroxidase (ceruloplasmin).
The kinetics of the catalysis of Fe(I1) oxidation by crystalline human ferroxidase (ceruloplasmin) has been studied under various conditions at 30’. Two Km values with respect to Fe(U), Km1 = 0.6 /.tM and Km2 = 50 PM, were obtained at pH 6.5, at which ferroxidase gave the maximum activity of 550 Fe(I1) per ferroxidase per min in an acetate buffer. Competition between the two substrates, p-pheny...
متن کاملPhysical and chemical studies on ceruloplasmin. V. Metabolic studies on sialic acid-free ceruloplasmin in vivo.
Injection into rabbits of radioactive ceruloplasmin from which sialic acid had been removed enzymatically resulted in a rapid disappearance of the asialoceruloplasmin from the seruni and its appearance in parenchymal cells, but not in Kupffer cells, of the liver. The rapidity of this transfer was dependent upon the presence of intact galactose residues as the terminal, nonreducing sugar of the ...
متن کاملIsotopic effect on the kinetic of thermal denaturation of ceruloplasmin.
The kinetics of thermal denaturation of ceruloplasmin in water and in water with different percentage of D2O in the temperature range 25-85 degrees C, following the optical density change of the 610 nm charge transfer band of the protein has been investigated. A temperature Tt approximately equal to 65 degrees C above which an irreversible denaturation process of the protein active site takes p...
متن کاملPhysical and Chemical Studies on Ceruloplasmin. Ii. Molecular Oxygen and the Blue Color of Ceruloplasmin.
In their description of the isolation and properties of ceruloplasmin, Holmberg and Laurel1 (1) noted that this intensely blue copper-protein could be decolorized by reducing agents such as ascorbic acid. Although the decoloriaation was “. . . completely reversible in the presence of oxygen, attempts to (restore blue color by oxidation) with potassium ferricyanide were not successful.” They won...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1971
ISSN: 0014-5793
DOI: 10.1016/0014-5793(71)80133-3